A Team of Researchers at the University Medical Center Schleswig-Holstein (UKSH), Kiel Campus, The Kiel University, and the Max Planck Institute for Molecular Genetics, Berlin, Berly, Berlin, Eve Developed Annovatti Method for real-time molecular genetic classification of brain tumors during surgery. This Approach Combines DNA Methylation Analysis with Advanced Machine Learning Technologies to Provide Detailed Information About The Tumor Type during Surgery.

For the first time, neurosurgery can be tailored not only to the location and proximity of a tumor to critical brain functions but also to its specific molecular characteristics. This enables a targeted approach to tumor removal, which is a decisive advantage for patients, especially in complex cass. The Findings Were published on 28 February 2025 in Nature Medicine,

“ONELY Through Close collaboration between basic research scientists and physicians working in translational medicine was it possible to develop a method that surpasses all previous SIMILARUOUs SIMILARUOUPASSES Precision and Speed, “Says Prof. Dr. Franz-Josef Müller, Deputy Director of the Department of Psychiatry and Psychotherapy at the Uksh, Kiel Campus, and Professor in the Medical Faculty of the Cau.

He Led the Interdisciplinary Team Togetra with Prof. Dr. Helene Kretzmer, Former Group Leader “Computational Genomics” at the MPIMG, Dr. Alena Van Bömmel, Former Postdoc at the MPIMG, Ph.D. Student Mara Steiger, MPIMG, and Ph.D. Student Björn Brändl, Center for Integrative Psychiatry, Campus Kiel.

The Study was conducted in collaboration with the department of neurosurgery at uksh, campus kiel. “While Past Advances in Neurosurgery Have Refined Techniques For Tumor Removal, this disease-consumed approach revolutionizes the entrepreneur. Instead of simply focusing on the tumor ‘ Intraoperative identification of the tumor type opens up new passibilites for real-time adjustments in the surgical strategy based on the tumor’s specific biology.

“This Approach Directly Links Precision Medicine With Neurosurgery, Elevating Treatment from a Technique-Driven Procedure to a personalized, disease-consistent therapy, in this way, in this way Custom-Tailored Therapy, “Says Dr. Carolin Kubelt-Kwamin, Senior Physician at the Clinic for Neurosurgery.

DNA Methylation, an epigenetic marker, services as a “fingerprint” that reflects a tumor’s origin. Different types of tumors exhibit distinct methylation patterns, which can be analyzed via sequencing. The new method employees nanopore sequencing to identify these patterns with remarkable speed and accuracy.

“However, because this sequencing must occur within the narrow timeframe of surgery, it can realistically only only only Capture a Fraction of the Tumor’s Methylation Patterns,” Epolains Dr. Kretzmer. To address this limitation, the resultars applied a mathematical approach from Provibility theorem ‘Theorem.

“By using this method, we trained a machine learning model that, in combination with specialized software, processes the sequencing data in real time and enables tumor classification in lessation in les Than 0.1% of the genetic data, “Says mara steiger. This represents a significant advancement over previous methods, which took hours to weeks.

The study demonstrates that the results from this new method align with that the auropathological examination. The Researchers Highlight that the method can accurately classified Diagnostically Challenging Tumors, where traditional hastopathological techniques ofteen fall short shortage. This innovation offers a significant Advantage for Patients, Particularly in Complex Cases, by enabling a more targeted and precise approach to tumor removal.

Modern molecular and epigenetic analyses have revised that many tumors previously thought to be one type of cancer are, in fact, highly divese. For example, tumors of the Central Nervous System are now Classified Into Nearly 90 Distinct Categories. These differenties Demand different therapeutic strategies. While some tumors can be treated with radiation or medicine, others requires extended surgery.

Until now, however, tumor tissue was only analyzed postoperatively, leaving surgeons uncertain about the tumor’s classification during surgery and forcing them to make decisions Brain tissue. Intraoperative DNA Methylation Analysis, Combined with Nanopore Sequencing, Now Provides Critical Information That Srigons Can Use in Real Time to Make moreMed Decisions, Supporting Personalized Decisions Precision Surgery.